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KMID : 1161520100140040261
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Animal Cells and Systems
2010 Volume.14 No. 4 p.261 ~ p.266
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Butyrate-induced differentiation of PC12 cells to chromaffin cells involves cell adhesion and induction of extracellular proteins and cell adhesion proteins
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Heo Jee-In
Oh Soo-Jin
Kho Yoon-Jung
Kim Jeong-Hyeon
Kang Hong-Joon
Park Seong-Hoon
Kim Hyun-Seok
Shin Jong-Yeon
Lee Sung-Young
Kim Min-Ju
Min Bon-Hong
Kim Sung-Chan
Park Jae-bong
Kim Jae-Bong
Lee Jae-Yong
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Abstract
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PC12 cells were differentiated into the cells of chromaffin phenotype by butyrate treatment. Cells were aggregated and formed tight cell adhesion. To investigate the molecular change in this differentiation, we examined expression levels of cell adhesion proteins and extracellular proteins during butyrate induced-differentiation of PC12 cells. Integrin β1, integrin α7, E cadherin, VCAM, collagen-I, fibronectin, desmoglein and connexin were increased during differentiation. The levels of clusterin and secreted clusterin were also increased. These increased levels of cell adhesion proteins and extracellular proteins appear to induce cell aggregation and tight cell adhesion. The levels of p21, p27 and p16 were increased probably because of differentiation-related growth arrest during differentiation. Prolonged incubation of butyrate up to 1 day was required for differentiation. Signal transduction pathways for this differentiatiom could not be identified since various inhibitors had no effect. The results showed that butyrate-induced differentiation of PC12 cells to chromaffin cells involves tight cell adhesion and induction of extracellular proteins and cell adhesion proteins.
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KEYWORD
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butyrate, PC12, differentiation, chromaffin cells, cell adhesion proteins
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